Development and Verification of Rapid Viability Polymerase Chain Reaction (RV-PCR) Protocols for Bacillus anthracis – For Application to Air Filters, Water, and Surface Samples

This report provides a detailed account of the development and verification of the RV-PCR method for detection of live spores of Bacillus anthracis Ames in environmental sample matrices such as the BioWatch air filters, surface sampling wipes, and water. NHSRC has made this publication available to assist in preparing for and recovering from disasters involving anthrax spores contamination. This work specifically represents a very important step in NHSRC’s support for the ERLN. It is also key to the Agency’s commitment to fulfill its homeland security mission and its overall mission to protect human health and the environment.

Author:
U.S. Environmental Protection Agency
Publish Date:
March 2011 (Revised)